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믹서 밀 MM 400 진정한 다목적 분쇄기

믹서 밀 MM 400
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The Mixer Mill MM 400 is a true multipurpose mill designed for dry, wet and cryogenic grinding of small volumes up to 2 x 20 ml. It mixes and homogenizes powders and suspensions with a frequency of 30 Hz within seconds – unbeatably fast and easy to operate.

The compact benchtop unit is suitable for classic homogenization processes, as well as for biological cell disruption for DNA/RNA and protein extraction. Long processing times up to 99 hours make the MM 400 ideally suited for research applications, for example in mechanochemistry.

With regard to performance and flexibility of this mill, there is no equivalent technology available in the market.

You may also be interested in the mixer mill models MM 500 nano and MM 500 vario which operate with the same functional principle at a frequency of 35 Hz but provide substantially higher performance. For applications which require cooling or heating the sample, the Mixer Mill MM 500 control is the perfect choice. Each RETSCH mixer mill has a specific application focus.

"We use the MM 400 to homogenize muscle, skin, liver, whole invertebrate samples prior to stable isotope and fatty acid analysis. It's fast and does a consistently thorough job of homogenizing samples. The machine has been heavily used with no mechanical issues."

Bruno Rosenberg

Fisheries and Oceans Canada

"Great for homogenous powdering of tissue samples whilst frozen"

Kristen Cooke

University of Sydney

"Perfect for preparing soil and plant material for isotope analysis."

David Mitchem

Virginia Tech

"The world's most effective instrument in medical technology. "

Muhammad Naveed

First Global Link

"I am happy to use MM 400 for my live laboratory. It would be specified as a standard machine for XRF marketplaces. "

Gye Ryoung Lee

Gachon University

"As always, excellent machine, easy to operate and user friendly. Fast milling to desired particulate size."

Hanna Kaliada

Vivex Biologics, Inc.

"This is the best ball mill I have ever used. Very easy to operate and handle."

Xinle Li
Clark Atlanta University

"I have used this machine in IISER Kolkata, India. It's very efficient, handy and convenient. It's worth buying it."

Surojit Bhunia

Northwestern University

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성능 및 디자인

  • 최대 30 Hz와 충격력 및 마찰력이 사용된 강력한 분쇄와 균질화
  • 회당 최대 20 개의 시료 작업이 가능한 2 개의 분쇄 스테이션 장착
  • Memory for 12 Standard Operating Procedures (SOP) and 6 program cycles
  • Convenient touch display, significant noise reduction

타의 추종을 불허하는 범용성

  • 3 가지 분쇄 모드 : 건식, 습식 또는 냉동
  • 예를 들어 XRF 분석을 위한 펠렛화 전 플라스틱 용기에서 분말 시료와 바인더를 혼합합니다.
  • 기계 화학과 같은 응용 분야 또는 볼 분쇄를 통한 생물학적 시료 파괴에 적합합니다.
  • Extraction of pesticides (QuEChERS) and herbal ingredients

믹서 밀 MM 400 Calibration ensures reproducible results

Reproducibility is paramount in the process chain from sampling to analysis. Lab equipment which can be calibrated guarantees reproducible results with minimum standard deviation every time. This is particularly useful when comparing results produced at different locations.

The MM 400 is the first laboratory mill which can be calibrated. RETSCH initially calibrates time and frequency of the mill and offers a regular calibration service to ensure reproducible milling processes.

This functionality is particularly suitable for

  • Testing labs with different locations
  • Accredited labs applying ISO/IEC 17025 or ISO 9000ff
  • Pharmaceutical products

믹서 밀 MM 400 생물학적 응용과 세포 파괴에 대한 솔루션

Mixer mills are frequently used for homogenizing biological samples. The so-called bead beating with small glass beads is an established method for cell disruption of yeasts, microalgae or bacteria. The sample is only moderately warmed in the process which can be reduced to a minimum by pre-cooling.

The MM 400 allows for efficient cell disruption of up to 240 ml cell suspension for DNA/RNA and protein extraction. For accurate diagnosis of infections, it is possible to isolate intact bacteria from tissue in 8 x 30 ml bottles or 10 x 5 ml vials by using adapters.

The MM 400 can be operated with a range of adapters for single-use vials with the following capacities:

20 x 0.2 ml / 20 x 1.5 or 2 ml / 10 x 5 ml / 8 x 30 ml / 8 x 50 ml

For the pulverization of 25 to 30 g plant material, such as cannabis flower, conical centrifuge tubes are best suited. Up to 8 tissue samples, like fresh liver in buffer solution, can also be homogenized in these 50 ml tubes using steel or zirconium oxide balls. To keep the mechanical stress on the vials as low as possible, a reduced frequency and a high filling level, e. g. with buffer and sample, are recommended.

 

믹서 밀 MM 400 - 생물학적 응용과 세포 파괴에 대한 솔루션

믹서 밀 MM 400 - 이스트 세포 파괴*

*The video shows the previous model with identical functional principle.

믹서 밀 MM 400 - Homogenization of cannabis*

The CryoKit is a cost-effective solution for cryogenic sample processing with the Mixer Mill MM 400. The set consists of two insulated containers, two tongs and safety glasses.

The sample to be embrittled and the grinding ball are filled into the stainless-steel grinding jar which is tightly screwed. Indirect embrittlement is effected by pre-cooling the jar in a liquid nitrogen bath. After approximately 2 minutes, the sample is sufficiently cooled for cryogenic processing.

If direct contact with liquid nitrogen is to be avoided, the CryoMill or Mixer Mill MM 500 control are suitable options. Both mills can be operated with jars made of other materials than steel for cryogenic grinding.

Vials, bottles and tubes available for MM 400

믹서 밀 MM 400 Recommended jar fillings

The jar size should be adapted to the sample volume to ensure optimum results. Ideally, the grinding balls are 3 times the size of the largest sample piece. The numbers and sizes of balls given in the table below follow this rule of thumb. To pulverize, for example, 20 ml of a sample consisting of 8-mm sized particles, the use of a 50 ml jar and 25 mm balls is recommended. According to the table, one grinding ball is required. 20 ml of a sample with 5-mm particles, however, can be homogenized with four 15 mm balls.

Fibrous: Hair

머리카락

30 ml sample
50 ml stainless steel jar
1 x 25 mm stainless steel ball
2 min at 30 Hz

Cell disruption: Microalgae

세포 파괴

30 ml cell suspension
8 x 50 ml conical centrifuge tubes (adapter)
with 25 ml glass beads each; 0,5-0,75 mm
30 s at 30 Hz

Elastic-Liquid:
Capsules with liquid

캡슐

15 ml sample
50 ml stainless steel jar
1 x 25 mm stainless steel ball
embrittlement in LN2 for 3 min
4 x 2 min at 30 Hz with intermediate cooling

Medium-hard/ fibrous: soil

 토양

20 ml sample
50 ml stainless steel jar
1 x 25 mm stainless steel ball
1 min at 30 Hz

Tough-Fibrous: Wood

5 ml sample
10 ml zirconium oxide jar
2 x 12 mm zirconium oxide balls
3 min at 30 Hz

Elastic-tough:
Polyurethane Pellets

20 ml sample
50 ml stainless steel jar
1 x 25 mm stainless steel ball
embrittlement in LN2 for 3 min
4 x 2 min at 30 Hz with intermediate cooling

Fibrous: Cannabis

3 g sample
50 ml stainless steel jar
1 x 25 mm stainless steel ball
embrittlement with LN2 for 2 min
90 s at 30 Hz

Hard-Brittle: Concrete

10 ml sample
25 ml zirconium oxide jar
2 x 15 mm zirconium oxide balls
2 min at 30 Hz

샘플 준비 작업에 가장 적합한 솔루션을 찾으려면 애플리케이션 데이터베이스를 방문하십시오.

믹서 밀 MM 400 작동 원리

믹서 밀 MM 400의 분쇄 용기는 수평 위치에서 방사형 진동을 수행합니다. 분쇄용 볼의 관성은 용기의 둥근 끝 부분에서 높은 충격 에너지를 발생시켜 시료를 분쇄합니다. 또한 분쇄 용기의 운동운동은 볼의 운동과 조합되어 시료의 집중 혼합을 발생시킵니다.

혼합 정도는 여러개의 작은 볼들을 사용하여 더욱 증가될 수 있습니다. 여러 개의 작은 볼들이 사용되는 경우 (예. 유리 재질 볼) 생물학적 세포가 파괴될 수 있습니다. 볼 사이의 큰 마찰 충격 효과가 효과적인 세포 파괴를 보장합니다.

기술적 변경 및 오차가 있을 수 있습니다